Dihydrofolate reductase
Details
- Name
- Dihydrofolate reductase
- Synonyms
- 1.5.1.3
- Gene Name
- DHFR
- Organism
- Humans
- Amino acid sequence
>lcl|BSEQ0036993|Dihydrofolate reductase MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFS IPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSS VYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKF EVYEKND
- Number of residues
- 187
- Molecular Weight
- 21452.61
- Theoretical pI
- 7.6
- GO Classification
- Functionsdihydrofolate reductase activity / drug binding / folic acid binding / methotrexate binding / mRNA binding / NADPH bindingProcessesaxon regeneration / dihydrofolate metabolic process / folic acid metabolic process / G1/S transition of mitotic cell cycle / glycine biosynthetic process / mitotic cell cycle / nitric oxide metabolic process / nucleotide biosynthetic process / one-carbon metabolic process / oxidation-reduction process / positive regulation of nitric-oxide synthase activity / regulation of nitric-oxide synthase activity / regulation of removal of superoxide radicals / regulation of transcription involved in G1/S transition of mitotic cell cycle / response to methotrexate / small molecule metabolic process / tetrahydrobiopterin biosynthetic process / tetrahydrofolate biosynthetic process / tetrahydrofolate metabolic process / vitamin metabolic process / water-soluble vitamin metabolic processComponentscytosol / nucleoplasm
- General Function
- Nadph binding
- Specific Function
- Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
- Pfam Domain Function
- DHFR_1 (PF00186)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016067|Dihydrofolate reductase (DHFR) ATGGTTGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAAC GGGGACCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACA ACCTCTTCAGTAGAAGGTAAACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCC ATTCCTGAGAAGAATCGACCTTTAAAGGGTAGAATTAATTTAGTTCTCAGCAGAGAACTC AAGGAACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTCTAGATGATGCCTTAAAACTT ACTGAACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGGTGGCAGTTCT GTTTATAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATG CAAGACTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTG CCAGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTT GAAGTATATGAGAAGAATGATTAA
- Chromosome Location
- 5
- Locus
- 5q11.2-q13.2
- External Identifiers
Resource Link UniProtKB ID P00374 UniProtKB Entry Name DYR_HUMAN GenBank Protein ID 182724 GenBank Gene ID J00140 GenAtlas ID DHFR HGNC ID HGNC:2861 - General References
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- Stockman BJ, Nirmala NR, Wagner G, Delcamp TJ, DeYarman MT, Freisheim JH: Sequence-specific 1H and 15N resonance assignments for human dihydrofolate reductase in solution. Biochemistry. 1992 Jan 14;31(1):218-29. [Article]
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Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB00563 Methotrexate approved yes inhibitor Details DB01157 Trimetrexate approved, investigational yes inhibitor Details DB00157 NADH approved, nutraceutical unknown Details DB00642 Pemetrexed approved, investigational yes inhibitor Details DB01131 Proguanil approved yes inhibitor Details DB02104 2,4-Diamino-5-Methyl-6-[(3,4,5-Trimethoxy-N-Methylanilino)Methyl]Pyrido[2,3-D]Pyrimidine experimental unknown Details DB02559 6-(Octahydro-1h-Indol-1-Ylmethyl)Decahydroquinazoline-2,4-Diamine experimental unknown Details DB02583 N6-(2,5-Dimethoxy-Benzyl)-N6-Methyl-Pyrido[2,3-D]Pyrimidine-2,4,6-Triamine experimental unknown Details DB02919 2,4-Diamino-6-[N-(3',4',5'-Trimethoxybenzyl)-N-Methylamino]Pyrido[2,3-D]Pyrimidine experimental unknown Details DB03060 Sri-9662 experimental unknown Details DB03351 Sri-9439 experimental unknown Details DB03461 Nicotinamide adenine dinucleotide phosphate experimental unknown Details DB03695 Piritrexim investigational unknown Details DB00205 Pyrimethamine approved, investigational, vet_approved yes inhibitor Details DB03886 Biopterin experimental unknown Details DB06358 Iclaprim investigational unknown Details DB07140 5-[(3R)-3-(5-methoxybiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine experimental unknown Details DB07141 5-[(3R)-3-(5-methoxy-4'-methylbiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine experimental unknown Details DB07142 5-[(3R)-3-(5-methoxy-3',5'-dimethylbiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine experimental unknown Details DB07144 5-[(3R)-3-(5-methoxy-2',6'-dimethylbiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine experimental unknown Details DB02427 2,4-Diamino-6-[N-(2',5'-Dimethoxybenzyl)-N-Methylamino]Quinazoline experimental unknown Details DB03987 2,4-Diamino-6-[N-(3',5'-Dimethoxybenzyl)-N-Methylamino]Pyrido[2,3-D]Pyrimidine experimental unknown Details DB08234 5-[3-(2,5-dimethoxyphenyl)prop-1-yn-1-yl]-6-ethylpyrimidine-2,4-diamine experimental unknown Details DB08406 [N-(2,4-DIAMINOPTERIDIN-6-YL)-METHYL]-DIBENZ[B,F]AZEPINE experimental unknown Details DB08448 (4aS)-5-[(2,4-diaminopteridin-6-yl)methyl]-4a,5-dihydro-2H-dibenzo[b,f]azepin-8-ol experimental unknown Details DB08642 (2R,6S)-6-{[methyl(3,4,5-trimethoxyphenyl)amino]methyl}-1,2,5,6,7,8-hexahydroquinazoline-2,4-diamine experimental unknown Details DB03125 2,4-Diamino-5-(3,4,5-Trimethoxy-Benzyl)-Pyrimidin-1-Ium experimental unknown Details DB00563 Methotrexate approved unknown substrate Details DB06813 Pralatrexate approved, investigational yes substrateinhibitor Details DB08878 Aminopterin investigational, withdrawn unknown inhibitor Details DB00798 Gentamicin approved, vet_approved unknown Details DB00555 Lamotrigine approved, investigational unknown inhibitor Details DB00158 Folic acid approved, nutraceutical, vet_approved no substrate Details