Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa.
Article Details
- CitationCopy to clipboard
Wilson SA, Chayen NE, Hemmings AM, Drew RE, Pearl LH
Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa.
J Mol Biol. 1991 Dec 20;222(4):869-71.
- PubMed ID
- 1762155 [ View in PubMed]
- Abstract
The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4(2)2(1)2 (a = 104.4 A, c = 66.6 A) with one subunit in the asymmetric unit. Crystals diffract beyond 2.8 A.