Amino acid sequence determination of guanyl-specific ribonuclease Sa from Streptomyces aureofaciens.
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Shlyapnikov SV, Both V, Kulikov VA, Dementiev AA, Sevcik J, Zelinka J
Amino acid sequence determination of guanyl-specific ribonuclease Sa from Streptomyces aureofaciens.
FEBS Lett. 1986 Dec 15;209(2):335-9.
- PubMed ID
- 3098582 [ View in PubMed]
- Abstract
Using automated Edman degradation of two nonfractionated peptide mixtures of tryptic and staphylococcal protease digests of the protein, the complete amino acid sequence of the guanyl-specific ribonuclease Sa from Streptomyces aureofaciens was established. Ribonuclease Sa contains 96 amino acid residues (Mr 10,566). A 50% sequence homology of ribonuclease Sa to the guanyl-specific ribonuclease St from S. erythreus was found.