Identification of the N-terminal residue of the heavy chain of both native and recombinant human hepatocyte growth factor.
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Yoshiyama Y, Arakaki N, Naka D, Takahashi K, Hirono S, Kondo J, Nakayama H, Gohda E, Kitamura N, Tsubouchi H, et al.
Identification of the N-terminal residue of the heavy chain of both native and recombinant human hepatocyte growth factor.
Biochem Biophys Res Commun. 1991 Mar 15;175(2):660-7.
- PubMed ID
- 1826837 [ View in PubMed]
- Abstract
The N-terminal amino acid of the heavy chain of native (purified from human plasma) and recombinant human hepatocyte growth factor (hHGF) was determined by analyses of amino acid composition and sequence of peptide fragments derived by enzymatic cleavage, peptide mapping, and fast atom bombardment mass spectrometry. Our results indicate that the N-terminal amino acid of the heavy chain of hHGF, both native and recombinant, is pyroglutamate, derived from glutamine at the 32nd residue from the initiation methionine.