Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Article Details
- CitationCopy to clipboard
Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Nature. 1985 Feb 28-Mar 6;313(6005):762-6.
- PubMed ID
- 3883192 [ View in PubMed]
- Abstract
The 3.3-A resolution crystal structure of the large proteolytic fragment of Escherichia coli DNA polymerase I complexed with deoxythymidine monophosphate consists of two domains, the smaller of which binds zinc-deoxythymidine monophosphate. The most striking feature of the larger domain is a deep crevice of the appropriate size and shape for binding double-stranded B-DNA. A flexible subdomain may allow the enzyme to surround completely the DNA substrate, thereby allowing processive nucleotide polymerization without enzyme dissociation.