Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.
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Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL
Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.
Nucleic Acids Res. 1993 Nov 25;21(23):5408-17.
- PubMed ID
- 8265357 [ View in PubMed]
- Abstract
We present the sequence of 176 kilobases of the Escherichia coli K-12 genome, from katG at 89.2 to an open reading frame (ORF) of unknown function at 92.8 minutes on the genetic map. This brings the total of contiguous sequence from the E. coli genome project to 500 kb (81.5 to 92.8 minutes). This segment contains 134 putative coding genes (ORFs) of which 66 genes were previously identified. Eight new genes--acs, pepE, and nrfB-G--were identified as well as the previously mapped gldA and alr genes. Still, 58 ORFs remain unidentified despite literature and similarity searches. The arrangement of proposed genes relative to possible promoters and terminators suggests 55 potential transcription units. Other features include 13 REP elements, one IRU (ERIC) repeat, 59 computer-predicted bends, 42 Chi sites and one new grey hole. Sixteen signal peptides were found, including those of lamB, btuB, and malE. Two ribosomal RNA loci, rrnB and rrnE, are located in this segment, so we have now sequenced four of the seven E. coli rRNA loci. Comparison of the rRNA loci reveals some differences in the ribosomal structural RNAs which are generally compatible with the proposed secondary structures.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID DNA-directed RNA polymerase subunit beta P0A8V2 Details Pantothenate kinase P0A6I3 Details Formate dehydrogenase H P07658 Details Vitamin B12 transporter BtuB P06129 Details Chorismate pyruvate-lyase P26602 Details Acetylglutamate kinase P0A6C8 Details Methionine synthase P13009 Details Phosphoenolpyruvate carboxylase P00864 Details Maltose-binding periplasmic protein P0AEX9 Details Class B acid phosphatase P0AE22 Details Cytochrome c-552 P0ABK9 Details Single-stranded DNA-binding protein P0AGE0 Details Hydrogen peroxide-inducible genes activator P0ACQ4 Details UDP-N-acetylenolpyruvoylglucosamine reductase P08373 Details Maltoporin P02943 Details Bifunctional ligase/repressor BirA P06709 Details DNA-directed RNA polymerase subunit beta' P0A8T7 Details UvrABC system protein A P0A698 Details