The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Murray J, Zhang B, Taylor SW, Oglesbee D, Fahy E, Marusich MF, Ghosh SS, Capaldi RA
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
J Biol Chem. 2003 Apr 18;278(16):13619-22. Epub 2003 Feb 28.
- PubMed ID
- 12611891 [ View in PubMed]
- Abstract
Defects of the NADH dehydrogenase complex are predominantly manifested in mitochondrial diseases and are significantly associated with the development of many late onset neurological disorders such as Parkinson's disease. Here we describe an immunocapture procedure for isolating this multisubunit membrane-bound complex from human tissue. Using small amounts of immunoisolated protein, one-dimensional and two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) peptide mass finger printing (PMF), and nanoflow liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS), we can resolve and identify the human homologues of 42 polypeptides detected so far in the more extensively studied beef heart complex I. These polypeptides include the GRIM-19 protein, which is claimed to be involved in apoptosis, a polypeptide first identified by gene screening as a neuronal protein, as well as a protein thought to be in differentiation linked processes. The concordance of data from human and bovine complex I isolated by different procedures adds to the certainty that these novel proteins of seemingly diverse function are a part of complex I.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial P56181 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial O00217 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial O95299 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 O43920 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial O75380 Details NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial P19404 Details Cytochrome c oxidase subunit NDUFA4 O00483 Details NADH dehydrogenase [ubiquinone] 1 subunit C2 O95298 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial O75489 Details NADH-ubiquinone oxidoreductase chain 3 P03897 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 P56556 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 O15239 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial O43674 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 O96000 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 O43676 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 O95168 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Q16795 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial O75251 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 O75438 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 P51970 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 O95139 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 O95182 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial O95178 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Q16718 Details NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial P49821 Details NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial P28331 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial O43181 Details NADH-ubiquinone oxidoreductase chain 1 P03886 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 P17568 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Q9P0J0 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Q86Y39 Details Acyl carrier protein, mitochondrial O14561 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 O43678 Details NADH-ubiquinone oxidoreductase chain 4 P03905 Details NADH-ubiquinone oxidoreductase chain 5 P03915 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Q9Y6M9 Details NADH-ubiquinone oxidoreductase chain 2 P03891 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial O95169 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 O95167 Details NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial O75306 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Q9UI09 Details