Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3).
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Race JE, Grassl SM, Williams WJ, Holtzman EJ
Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3).
Biochem Biophys Res Commun. 1999 Feb 16;255(2):508-14.
- PubMed ID
- 10049739 [ View in PubMed]
- Abstract
The cloned organic anion transporters from rat, mouse, and winter flounder (rOAT1, mOAT1, fROAT) mediate the coupled exchange of alpha-ketoglutarate with multiple organic anions, including p-aminohippurate (PAH). We have isolated two novel gene products from human kidney which bear significant homology to the known OATs and belong to the amphiphilic solute facilitator (ASF) family. The cDNAs, hOAT1 and hOAT3, encode for 550- and 568-amino-acid residue proteins, respectively. hOAT1 and hOAT3 mRNAs are expressed strongly in kidney and weakly in brain. Both genes map to chromosome 11 region q11.7. PAH uptake by Xenopus laevis oocytes injected with hOAT1 mRNA is increased 100-fold compared to water-injected oocytes. PAH uptake is chloride dependent and is not further increased by preincubation of oocytes in 5 mM glutarate. Uptake of PAH is inhibited by probenicid, alpha-ketoglutarate, bumetanide, furosemide, and losartan, but not by salicylate, urate, choline, amilioride, and hydrochlorothiazide.
DrugBank Data that Cites this Article
- Drug Transporters
Drug Transporter Kind Organism Pharmacological Action Actions Aminohippuric acid Solute carrier family 22 member 8 Protein Humans UnknownSubstrateInhibitorDetails Bumetanide Solute carrier family 22 member 6 Protein Humans UnknownSubstrateInhibitorDetails Hydrochlorothiazide Solute carrier family 22 member 6 Protein Humans UnknownSubstrateInhibitorDetails Losartan Solute carrier family 22 member 6 Protein Humans UnknownInhibitorDetails Oxalic Acid Solute carrier family 22 member 8 Protein Humans UnknownSubstrateDetails Probenecid Solute carrier family 22 member 6 Protein Humans UnknownSubstrateInhibitorDetails Salicylic acid Solute carrier family 22 member 6 Protein Humans UnknownInhibitorDetails - Polypeptides
Name UniProt ID Solute carrier family 22 member 6 Q4U2R8 Details Solute carrier family 22 member 8 Q8TCC7 Details