A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases.
Article Details
- CitationCopy to clipboard
Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, Fita I, Loewen PC
A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases.
EMBO Rep. 2005 Dec;6(12):1156-62.
- PubMed ID
- 16211084 [ View in PubMed]
- Abstract
The catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. An arginine, 20 A from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by H(2)O(2), facilitating the catalatic pathway in a peroxidase. The influence of the arginine is imparted to the heme through its association with or dissociation from a tyrosinate that modulates reactivity through a Met-Tyr-Trp crosslinked adduct and a pi electron interaction of the heme with the adduct Trp.