6-deoxyerythronolide B hydroxylase
Details
- Name
- 6-deoxyerythronolide B hydroxylase
- Synonyms
- 1.14.13.188
- 6-DEB hydroxylase
- CYP107A1
- CYPCVIIA1
- Cytochrome P450 107A1
- Cytochrome P450eryF
- Erythromycin A biosynthesis hydroxylase
- Gene Name
- eryF
- Organism
- Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
- Amino acid sequence
>lcl|BSEQ0011165|6-deoxyerythronolide B hydroxylase MTTVPDLESDSFHVDWYRTYAELRETAPVTPVRFLGQDAWLVTGYDEAKAALSDLRLSSD PKKKYPGVEVEFPAYLGFPEDVRNYFATNMGTSDPPTHTRLRKLVSQEFTVRRVEAMRPR VEQITAELLDEVGDSGVVDIVDRFAHPLPIKVICELLGVDEKYRGEFGRWSSEILVMDPE RAEQRGQAAREVVNFILDLVERRRTEPGDDLLSALIRVQDDDDGRLSADELTSIALVLLL AGFEASVSLIGIGTYLLLTHPDQLALVRRDPSALPNAVEEILRYIAPPETTTRFAAEEVE IGGVAIPQYSTVLVANGAANRDPKQFPDPHRFDVTRDTRGHLSFGQGIHFCMGRPLAKLE GEVALRALFGRFPALSLGIDADDVVWRRSLLLRGIDHLPVRLDG
- Number of residues
- 404
- Molecular Weight
- 45098.685
- Theoretical pI
- 4.69
- GO Classification
- Functionsheme binding / iron ion binding / monooxygenase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygenProcesseserythromycin biosynthetic process / oxidation-reduction processComponentscytoplasm
- General Function
- Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
- Specific Function
- Catalyzes the NADPH-dependent conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the monooxygenase.
- Pfam Domain Function
- p450 (PF00067)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0011166|6-deoxyerythronolide B hydroxylase (eryF) ATGACGACCGTTCCCGATCTCGAAAGCGACTCCTTCCACGTCGACTGGTACCGCACCTAC GCCGAGCTGCGCGAGACCGCGCCGGTGACGCCGGTGCGCTTCCTCGGCCAGGACGCGTGG CTGGTCACCGGCTACGACGAGGCGAAGGCCGCGCTGAGCGACCTGCGCCTGAGCAGCGAC CCGAAGAAGAAGTACCCGGGCGTGGAGGTCGAGTTCCCGGCATACCTCGGTTTCCCCGAG GACGTGCGGAACTACTTCGCCACCAACATGGGCACCAGCGACCCGCCGACCCACACCCGG CTGCGCAAGCTGGTGTCGCAGGAGTTCACCGTCCGCCGCGTGGAGGCGATGCGGCCCCGC GTCGAGCAGATCACCGCGGAGCTGCTCGACGAGGTGGGCGACTCCGGCGTGGTCGACATC GTCGACCGCTTCGCCCACCCGCTGCCCATCAAGGTCATCTGCGAGCTGCTCGGCGTCGAC GAGAAGTACCGCGGGGAGTTCGGGCGGTGGAGCTCGGAGATCCTGGTCATGGACCCGGAG CGGGCCGAACAGCGCGGGCAGGCGGCCAGGGAGGTCGTCAACTTCATCCTCGACCTGGTC GAGCGCCGCCGCACCGAGCCCGGCGACGACCTGCTGTCCGCGCTGATCAGGGTCCAGGAC GACGATGACGGTCGGCTCAGCGCCGACGAGCTGACCTCCATCGCGCTGGTGCTGCTGCTG GCCGGTTTCGAGGCGTCGGTGAGCCTCATCGGGATCGGCACCTACCTGCTGCTCACCCAC CCGGACCAGCTCGCGCTGGTGCGGCGGGACCCGTCGGCGCTGCCCAACGCCGTCGAGGAG ATCCTGCGCTACATCGCTCCGCCGGAGACCACCACGCGCTTCGCCGCGGAGGAGGTGGAG ATCGGCGGTGTCGCGATCCCCCAGTACAGCACGGTGCTGGTCGCGAACGGCGCGGCCAAC CGCGACCCGAAGCAGTTCCCGGACCCCCACCGCTTCGACGTCACCCGCGACACCCGCGGC CACCTGTCGTTCGGGCAGGGCATCCACTTCTGCATGGGCCGGCCGCTGGCCAAGCTGGAG GGCGAGGTGGCGCTGCGGGCGCTGTTCGGCCGCTTCCCCGCTCTGTCGCTGGGAATCGAC GCCGACGACGTGGTGTGGCGGCGTTCGCTGCTGCTGCGGGGCATCGACCACCTACCGGTG CGGCTCGACGGATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q00441 UniProtKB Entry Name CPXJ_SACEN GenBank Protein ID 48942 GenBank Gene ID X60379 - General References
- Haydock SF, Dowson JA, Dhillon N, Roberts GA, Cortes J, Leadlay PF: Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol Gen Genet. 1991 Nov;230(1-2):120-8. [Article]
- Weber JM, Leung JO, Swanson SJ, Idler KB, McAlpine JB: An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. Science. 1991 Apr 5;252(5002):114-7. [Article]
- Oliynyk M, Samborskyy M, Lester JB, Mironenko T, Scott N, Dickens S, Haydock SF, Leadlay PF: Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338. Nat Biotechnol. 2007 Apr;25(4):447-53. Epub 2007 Mar 18. [Article]
- Andersen JF, Hutchinson CR: Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase. J Bacteriol. 1992 Feb;174(3):725-35. [Article]
- Cupp-Vickery JR, Poulos TL: Structure of cytochrome P450eryF involved in erythromycin biosynthesis. Nat Struct Biol. 1995 Feb;2(2):144-53. [Article]
- Cupp-Vickery J, Anderson R, Hatziris Z: Crystal structures of ligand complexes of P450eryF exhibiting homotropic cooperativity. Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3050-5. [Article]
- Cupp-Vickery JR, Garcia C, Hofacre A, McGee-Estrada K: Ketoconazole-induced conformational changes in the active site of cytochrome P450eryF. J Mol Biol. 2001 Aug 3;311(1):101-10. [Article]
- Nagano S, Cupp-Vickery JR, Poulos TL: Crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and its mutants, A245S and A245T: investigation of the proton transfer system in P450eryF. J Biol Chem. 2005 Jun 10;280(23):22102-7. Epub 2005 Apr 11. [Article]