4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase
Details
- Name
- 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase
- Synonyms
- 4-BUDH
- 4.2.1.120
- Gamma-aminobutyrate metabolism dehydratase/isomerase
- Gene Name
- abfD
- Organism
- Clostridium aminobutyricum
- Amino acid sequence
>lcl|BSEQ0022437|4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase MLMTAEQYIESLRKLNTRVYMFGEKIENWVDHPMIRPSINCVAMTYELAQDPQYADLMTT KSNLIGKTINRFANLHQSTDDLRKKVKMQRLLGQKTASCFQRCVGMDAFNAVFSTTYEID QKYGTNYHKNFTEYLKYIQENDLIVDGAMTDPKGDRGLAPSAQKDPDLFLRIVEKREDGI VVRGAKAHQTGSINSHEHIIMPTIAMTEADKDYAVSFACPSDADGLFMIYGRQSCDTRKM EEGADIDLGNKQFGGQEALVVFDNVFIPNDRIFLCQEYDFAGMMVERFAGYHRQSYGGCK VGVGDVVIGAAALAADYNGAQKASHVKDKLIEMTHLNETLYCCGIACSAEGYPTAAGNYQ IDLLLANVCKQNITRFPYEIVRLAEDIAGGLMVTMPSEADFKSETVVGRDGETIGDFCNK FFAAAPTCTTEERMRVLRFLENICLGASAVGYRTESMHGAGSPQAQRIMIARQGNINAKK ELAKAIAGIK
- Number of residues
- 490
- Molecular Weight
- 54421.685
- Theoretical pI
- 6.03
- GO Classification
- Functions4 iron, 4 sulfur cluster binding / 4-hydroxybutanoyl-CoA dehydratase activity / metal ion binding / oxidoreductase activity, acting on the CH-CH group of donors / vinylacetyl-CoA delta-isomerase activity
- General Function
- Vinylacetyl-coa delta-isomerase activity
- Specific Function
- Catalyzes the reversible conversion of 4-hydroxybutyryl-CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps: (1) the FAD-dependent oxidation of 4-hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substituted by a hydride derived from the now reduced FAD in an SN2' reaction leading to vinylacetyl-CoA; (3) isomerization to yield crotonyl-CoA.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0005817|1473 bp ATGTTAATGACAGCAGAACAGTACATTGAGAGTCTAAGAAAGCTAAACACAAGAGTTTAT ATGTTTGGTGAAAAAATCGAGAATTGGGTGGATCATCCAATGATCAGACCTTCCATCAAC TGCGTAGCAATGACTTATGAATTAGCTCAGGATCCTCAGTACGCTGACTTAATGACTACA AAGTCAAACTTAATAGGTAAAACTATCAACAGATTTGCAAATCTACACCAGAGCACAGAT GACCTTAGAAAAAAGGTTAAGATGCAGAGACTTCTTGGACAGAAGACCGCATCATGCTTC CAGAGATGTGTAGGTATGGACGCTTTCAATGCAGTTTTCTCAACTACATATGAAATCGAC CAGAAATATGGAACAAACTATCACAAGAACTTTACTGAATACTTAAAGTATATACAGGAA AATGACCTTATTGTTGACGGTGCAATGACTGACCCTAAGGGTGACAGAGGACTTGCTCCA TCCGCACAGAAGGATCCAGATCTTTTCTTGAGAATCGTTGAAAAAAGAGAAGATGGTATC GTTGTAAGAGGAGCTAAGGCTCACCAGACTGGTTCCATCAACTCCCACGAACACATCATC ATGCCTACAATCGCTATGACAGAAGCTGATAAGGATTATGCAGTATCATTTGCTTGTCCT TCCGATGCTGATGGTCTATTCATGATCTACGGCAGACAGTCATGTGACACAAGAAAGATG GAAGAAGGCGCTGACATTGACCTTGGTAACAAGCAGTTCGGCGGACAGGAAGCTTTAGTC GTATTCGATAACGTATTTATTCCAAATGACAGAATCTTCCTTTGCCAAGAATATGATTTC GCTGGCATGATGGTAGAAAGATTTGCTGGATACCACAGACAGTCATACGGCGGATGTAAG GTTGGAGTAGGCGACGTTGTAATCGGTGCTGCTGCTTTAGCTGCTGACTACAATGGAGCT CAGAAGGCTTCTCACGTTAAAGATAAGCTTATCGAAATGACTCACTTAAATGAAACTTTA TATTGCTGCGGTATTGCTTGTTCAGCAGAAGGTTATCCAACTGCTGCTGGTAACTATCAG ATTGACCTTCTTCTTGCAAATGTATGTAAGCAGAACATCACTAGATTCCCTTACGAAATC GTAAGACTAGCTGAAGATATCGCTGGTGGATTAATGGTTACTATGCCTTCAGAAGCTGAC TTTAAGTCAGAAACAGTTGTTGGTAGAGATGGCGAAACTATTGGAGATTTCTGCAATAAG TTCTTCGCTGCTGCTCCTACTTGCACAACAGAAGAAAGAATGAGAGTTCTTAGATTCTTA GAAAACATCTGCTTAGGTGCATCCGCTGTAGGTTACAGAACTGAATCCATGCATGGTGCA GGTTCCCCTCAGGCTCAGAGAATCATGATCGCTCGTCAGGGCAACATCAACGCTAAGAAA GAATTAGCTAAGGCAATCGCTGGAATTAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P55792 UniProtKB Entry Name HDVD_CLOAM GenBank Gene ID AJ250267 - General References
- Gerhardt A, Cinkaya I, Linder D, Huisman G, Buckel W: Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA. Arch Microbiol. 2000 Sep;174(3):189-99. [Article]
- Scherf U, Buckel W: Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum. Eur J Biochem. 1993 Jul 15;215(2):421-9. [Article]
- Martins BM, Dobbek H, Cinkaya I, Buckel W, Messerschmidt A: Crystal structure of 4-hydroxybutyryl-CoA dehydratase: radical catalysis involving a [4Fe-4S] cluster and flavin. Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15645-9. Epub 2004 Oct 20. [Article]