UDP-glucose 4-epimerase
Details
- Name
- UDP-glucose 4-epimerase
- Synonyms
- 5.1.3.2
- Galactowaldenase
- galD
- UDP-galactose 4-epimerase
- Gene Name
- galE
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0016603|UDP-glucose 4-epimerase MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGKHPTFVEGDIR NEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVNGTLRLISAMRAANVKNFI FSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQILTDLQKAQPDWSIALLRYFNP VGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLA DGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYW ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD
- Number of residues
- 338
- Molecular Weight
- 37264.875
- Theoretical pI
- 6.32
- GO Classification
- Functionsidentical protein binding / NAD+ binding / racemase and epimerase activity, acting on carbohydrates and derivatives / UDP-glucose 4-epimerase activityProcessescarbohydrate metabolic process / colanic acid biosynthetic process / galactose catabolic process via UDP-galactose / galactose metabolic processComponentscytoplasm / cytosol
- General Function
- Udp-glucose 4-epimerase activity
- Specific Function
- Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. It is only active on UDP-galactose and UDP-glucose.
- Pfam Domain Function
- GDP_Man_Dehyd (PF16363)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016604|UDP-glucose 4-epimerase (galE) ATGAGAGTTCTGGTTACCGGTGGTAGCGGTTACATTGGAAGTCATACCTGTGTGCAATTA CTGCAAAACGGTCATGATGTCATCATTCTTGATAACCTCTGTAACAGTAAGCGCAGCGTA CTGCCTGTTATCGAGCGTTTAGGCGGCAAACATCCAACGTTTGTTGAAGGCGATATTCGT AACGAAGCGTTGATGACCGAGATCCTGCACGATCACGCTATCGACACCGTGATCCACTTC GCCGGGCTGAAAGCCGTGGGCGAATCGGTACAAAAACCGCTGGAATATTACGACAACAAT GTCAACGGCACTCTGCGCCTGATTAGCGCCATGCGCGCCGCTAACGTCAAAAACTTTATT TTTAGCTCCTCCGCCACCGTTTATGGCGATCAGCCCAAAATTCCATACGTTGAAAGCTTC CCGACCGGCACACCGCAAAGCCCTTACGGCAAAAGCAAGCTGATGGTGGAACAGATCCTC ACCGATCTGCAAAAAGCCCAGCCGGACTGGAGCATTGCCCTGCTGCGCTACTTCAACCCG GTTGGCGCGCATCCGTCGGGCGATATGGGCGAAGATCCGCAAGGCATTCCGAATAACCTG ATGCCATACATCGCCCAGGTTGCTGTAGGCCGTCGCGACTCGCTGGCGATTTTTGGTAAC GATTATCCGACCGAAGATGGTACTGGCGTACGCGATTACATCCACGTAATGGATCTGGCG GACGGTCACGTCGTGGCGATGGAAAAACTGGCGAACAAGCCAGGCGTACACATCTACAAC CTCGGCGCTGGCGTAGGCAACAGCGTGCTGGACGTGGTTAATGCCTTCAGCAAAGCCTGC GGCAAACCGGTTAATTATCATTTTGCACCGCGTCGCGAGGGCGACCTTCCGGCCTACTGG GCGGACGCCAGCAAAGCCGACCGTGAACTGAACTGGCGCGTAACGCGCACACTCGATGAA ATGGCGCAGGACACCTGGCACTGGCAGTCACGCCATCCACAGGGATATCCCGATTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P09147 UniProtKB Entry Name GALE_ECOLI GenBank Protein ID 41523 GenBank Gene ID X06226 - General References
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- Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
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- Wilson DB, Hogness DS: The enzymes of the galactose operon in Escherichia coli. II. The subunits of uridine diphosphogalactose 4-epimerase. J Biol Chem. 1969 Apr 25;244(8):2132-6. [Article]
- Swanson BA, Frey PA: Identification of lysine 153 as a functionally important residue in UDP-galactose 4-epimerase from Escherichia coli. Biochemistry. 1993 Dec 7;32(48):13231-6. [Article]
- Liu Y, Vanhooke JL, Frey PA: UDP-galactose 4-epimerase: NAD+ content and a charge-transfer band associated with the substrate-induced conformational transition. Biochemistry. 1996 Jun 11;35(23):7615-20. [Article]
- Bauer AJ, Rayment I, Frey PA, Holden HM: The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolution. Proteins. 1992 Apr;12(4):372-81. [Article]
- Thoden JB, Frey PA, Holden HM: Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli. Biochemistry. 1996 Feb 27;35(8):2557-66. [Article]
- Thoden JB, Frey PA, Holden HM: Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism. Biochemistry. 1996 Apr 23;35(16):5137-44. [Article]
- Thoden JB, Frey PA, Holden HM: High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol. Protein Sci. 1996 Nov;5(11):2149-61. [Article]
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- Thoden JB, Henderson JM, Fridovich-Keil JL, Holden HM: Structural analysis of the Y299C mutant of Escherichia coli UDP-galactose 4-epimerase. Teaching an old dog new tricks. J Biol Chem. 2002 Jul 26;277(30):27528-34. Epub 2002 May 17. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB02421 Uridine-5'-diphosphate-mannose experimental unknown Details DB02790 Phenyl-uridine-5'-diphosphate experimental unknown Details DB03397 Uridine-Diphosphate-N-Acetylglucosamine experimental unknown Details DB03435 Uridine-5'-Diphosphate experimental unknown Details DB04097 Uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-D-galactose experimental unknown Details DB01861 Uridine diphosphate glucose experimental unknown Details