Enrichment of glycopeptides for glycan structure and attachment site identification.
Article Details
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Nilsson J, Ruetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G
Enrichment of glycopeptides for glycan structure and attachment site identification.
Nat Methods. 2009 Nov;6(11):809-11. doi: 10.1038/nmeth.1392. Epub 2009 Oct 18.
- PubMed ID
- 19838169 [ View in PubMed]
- Abstract
We present a method to enrich for glycoproteins from proteomic samples. Sialylated glycoproteins were selectively periodate-oxidized, captured on hydrazide beads, trypsinized and released by acid hydrolysis of sialic acid glycosidic bonds. Mass spectrometric fragment analysis allowed identification of glycan structures, and additional fragmentation of deglycosylated ions yielded peptide sequence information, which allowed glycan attachment site and protein identification. We identified 36 N-linked and 44 O-linked glycosylation sites on glycoproteins from human cerebrospinal fluid.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Prothrombin P00734 Details Antithrombin-III P01008 Details Serotransferrin P02787 Details Thyroxine-binding globulin P05543 Details Neuron-specific vesicular protein calcyon Q9NYX4 Details Alpha-1-acid glycoprotein 1 P02763 Details Alpha-1-antitrypsin P01009 Details Kininogen-1 P01042 Details Apolipoprotein E P02649 Details Ceruloplasmin P00450 Details Plasma protease C1 inhibitor P05155 Details Beta-2-glycoprotein 1 P02749 Details Monocyte differentiation antigen CD14 P08571 Details Alpha-1-acid glycoprotein 2 P19652 Details Complement C4-A P0C0L4 Details Apolipoprotein D P05090 Details Ig alpha-1 chain C region P01876 Details Apolipoprotein C-III P02656 Details Afamin P43652 Details Alpha-1B-glycoprotein P04217 Details Alpha-2-HS-glycoprotein P02765 Details Complement factor H P08603 Details Complement factor I P05156 Details Clusterin P10909 Details Leucine-rich alpha-2-glycoprotein P02750 Details Pigment epithelium-derived factor P36955 Details Amyloid-like protein 1 P51693 Details Insulin-like growth factor-binding protein 6 P24592 Details Prostaglandin-H2 D-isomerase P41222 Details Hemopexin P02790 Details Haptoglobin P00738 Details