Metabolism of a mutant insulin by a receptor-mediated process and an insulin degrading enzyme.

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Kobayashi M, Iwasaki M, Watanabe N, Ishibashi O, Takata Y, Haruta T, Sasaoka T, Shigeta Y, Inouye K

Metabolism of a mutant insulin by a receptor-mediated process and an insulin degrading enzyme.

Diabetes Res Clin Pract. 1986 Sep-Oct;2(5):257-62. doi: 10.1016/s0168-8227(86)80001-8.

PubMed ID

3536368 [ View in PubMed

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Abstract

The metabolism of a mutant insulin, [LeuB25]-insulin, was studied in vitro and in vivo. Porcine or mutant insulin (4 micrograms/kg body weight) was injected i.v. into streptozotocin-induced diabetic rats and their plasma glucose and insulin levels were determined. The half-lives of porcine and mutant insulin were 3 min and 18 min, respectively. The ability of the mutant insulin to lower the blood glucose levels was 38% of that of normal when the glucose levels at the nadir were compared. Receptor-mediated degradation of the mutant insulin assessed by chromatography of the degraded materials in the media after incubation with cells was less compared with that of porcine insulin (4% vs. 24%). The media containing the insulin degrading enzyme (IDE) of IM-9 cells and rat livers degraded porcine and mutant insulin to the same extent. These results suggest that the decreased clearance of insulin is due to the decreased receptor binding and the decreased receptor-mediated degradation, but is not due to the decreased degradation by IDE.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Insulin pork	Insulin-degrading enzyme	Protein	Humans	Unknown	Substrate	Details