Investigations into the sequence-selective binding of mithramycin and related ligands to DNA.

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Fox KR, Howarth NR

Investigations into the sequence-selective binding of mithramycin and related ligands to DNA.

Nucleic Acids Res. 1985 Dec 20;13(24):8695-714.

PubMed ID

2934687 [ View in PubMed

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Abstract

The preferred binding sites for mithramycin on four different DNA fragments have been investigated by DNAase I footprinting. Sites containing at least two contiguous GC base pairs are protected by the antibiotic, the preferred binding site consisting of the dinucleotide step GpG (or CpC). Related antibiotics chromomycin and olivomycin produce similar, but not identical footprinting patterns suggesting that they can recognize other sequences as well. All three antibiotics induce enhanced rates of enzyme cleavage at regions flanking some of their binding sites. These effects are generally observed in runs of A and T and are attributed to DNA structural variations induced in the vicinity of the ligand binding site. The reaction of dimethylsulphate with N7 of guanine was modified by the presence of mithramycin so that we cannot exclude the possibility that these antibiotics bind to DNA via the major groove.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Plicamycin	DNA	Nucleotide	Humans	Yes	Antagonist	Details