Nucleotide sequence of the pepN gene encoding aminopeptidase N of Escherichia coli.
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Foglino M, Gharbi S, Lazdunski A
Nucleotide sequence of the pepN gene encoding aminopeptidase N of Escherichia coli.
Gene. 1986;49(3):303-9.
- PubMed ID
- 2436977 [ View in PubMed]
- Abstract
We have sequenced a 3.3-kb fragment of the Escherichia coli chromosome that contains pepN gene encoding aminopeptidase N. This gene codes for a protein of 870 amino acid residues. From the size of the pepN transcript and the presence of inverted repeats in the nucleotide (nt) sequence, a putative transcription terminator has been identified. The N-terminal amino acid sequence deduced from the pepN nt sequence corresponds to the N-terminal sequence of the purified protein; the amino acid composition of the protein is also in good agreement with that deduced from the gene sequence. No obvious homology with previously sequenced peptidases has been detected.