beta-Ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue.

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Kauppinen S, Siggaard-Andersen M, von Wettstein-Knowles P

beta-Ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue.

Carlsberg Res Commun. 1988;53(6):357-70.

PubMed ID

3076376 [ View in PubMed

]

Abstract

The fabB gene of E. coli encoding beta-ketoacyl-ACP synthase I has been isolated by complementation and sequenced. The enzyme has been purified and its NH2-terminal residues sequenced. Identification of the active site was accomplished by tagging with 3H-cerulenin and radio sequencing of the region. Comparison of the deduced primary structures of the fabB gene product with the FAS2 gene product of Saccharomyces cerevisiae revealed the probable active site in chalcone synthases of higher plants.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Cerulenin	3-oxoacyl-[acyl-carrier-protein] synthase 1	Protein	Escherichia coli (strain K12)	Yes	Inhibitor	Details

Polypeptides

Name	UniProt ID
3-oxoacyl-[acyl-carrier-protein] synthase 1	P0A953	Details